platelet activation marker  (FineTest Biotech Inc)

Journal: British Journal of Pharmacology

Article Title: Inhibition of oxidative stress by apocynin attenuated chronic obstructive pulmonary disease progression and vascular injury by cigarette smoke exposure

doi: 10.1111/bph.16068

Figure Lengend Snippet: Cigarette smoke (CS)‐induced vascular oxidative stress and platelet aggregation were prevented by apocynin treatment. Mice were exposed to CS or room air (sham) for 8 weeks with i.p. injection of apocynin (5 mg·kg −1 ·day −1 ) or vehicle (saline). Representative immunofluorescence images of thoracic aorta section stained with 3‐nitrotyrosine (3‐NT; a), platelet surface marker (CD41; b), or platelet activation marker (CD62p; c), with positive staining in green and nuclei in blue (DAPI). White arrows indicate positive stains and the respective positive stains were quantified as described in methods. Data are expressed as mean + SEM (n = 8 mice per group) and analysed by two‐way ANOVA with multiple comparisons and Tukey post hoc test. * P < 0.05 denotes differences between the compared groups.

Article Snippet: For aortic immunostaining, specific primary antibodies were used to detect vascular endothelial nitric oxide synthase (eNOS ; anti‐NOS3 at 1:100 dilution; Thermo Fisher Scientific, USA), vascular peroxynitrite (anti‐3‐Nitrotyrosine [3‐NT] at 1:100 dilution; Thermofisher Scientific, NY, USA), platelet surface marker Integrin alpha 2b (anti‐CD41 at 1:100 dilution; Bioss Antibodies, MA, USA) and platelet activation marker p‐selectin (anti‐CD62p at 1:100 dilution; Bioss Antibodies, MA, USA).

Techniques: Injection, Saline, Immunofluorescence, Staining, Marker, Activation Assay

Journal: British Journal of Pharmacology

Article Title: Inhibition of oxidative stress by apocynin attenuated chronic obstructive pulmonary disease progression and vascular injury by cigarette smoke exposure

doi: 10.1111/bph.16068

Figure Lengend Snippet: Long term cigarette smoke (CS)‐induced loss of eNOS, vascular oxidative stress and platelet activation were attenuated by apocynin treatment. Mice were exposed to CS or room air (sham) for 8 weeks with i.p. injection of apocynin (5 mg·kg −1 ·day −1 ) or vehicle (saline). Representative immunofluorescence images of thoracic aorta section stained with eNOS (a), 3‐NT (b), platelet surface CD41 (c), or platelet activation CD62p (d), with positive staining in green and nuclei in blue (DAPI). White arrows indicate positive stains and the respective positive stains were quantified as described in methods. Data are expressed as mean + SEM (n = 8 mice per group) and analysed by two‐way ANOVA with multiple comparisons and Tukey post hoc test. * P < 0.05, denotes differences between the compared groups.

Article Snippet: For aortic immunostaining, specific primary antibodies were used to detect vascular endothelial nitric oxide synthase (eNOS ; anti‐NOS3 at 1:100 dilution; Thermo Fisher Scientific, USA), vascular peroxynitrite (anti‐3‐Nitrotyrosine [3‐NT] at 1:100 dilution; Thermofisher Scientific, NY, USA), platelet surface marker Integrin alpha 2b (anti‐CD41 at 1:100 dilution; Bioss Antibodies, MA, USA) and platelet activation marker p‐selectin (anti‐CD62p at 1:100 dilution; Bioss Antibodies, MA, USA).

Techniques: Activation Assay, Injection, Saline, Immunofluorescence, Staining